Extração e identificação cromatográfica de clorofilas: uma abordagem experimental no Ensino Médio com Epipremnum pinnatum

Abstract

This paper addresses the application of an approach experimental focused on the study of plant pigments, with an emphasis on chromatography and spectroscopy, as pedagogical tools in the teaching of Chemistry. The objective was to promote active and investigative learning among students of the Instituto Federal de Goiás – Campus Itumbiara, stimulating the understanding of biological processes, such as photosynthesis, and the identification of different pigments in plants. The methodology adopted involved an experimental approach that allowed students to experience the stages of pigment extraction, planar chromatography, and the analysis of results through questionnaires, both before and after the activity. The study, characterized by a didactic intervention, focused on the practical application of theoretical concepts in an interactive manner, promoting learning based on direct observation and reflection. The results showed that the students showed great interest in the activities and were able to understand the relevance of plant pigments in the process of photosynthesis, although some conceptual gaps remained, especially regarding the precise differentiation of pigments and the explanation of the physiological mechanisms of photosynthesis. The evaluation through questionnaires revealed that most students actively engaged, recognizing the importance of the content and the experimental method, although difficulties were also identified in associating some theoretical answers with practical results. In conclusion, the project demonstrated the effectiveness of investigative and experimental methodologies for teaching Chemistry, highlighting the importance of active approaches that integrate theory and practice to promote meaningful learning and the development of critical thinking. The study reinforces the need for continuous pedagogical adjustments to overcome conceptual difficulties and deepen the understanding of the topics covered.