Novo detector natural para medições em terapia fotodinâmica via design de experimentos (DOE)

Abstract

Therapy benefited by light stimuli such as detector used as photosensitizers incidente to the medium, called the photodynamic effect, it gets the name photodynamic therapy (PDT). Mechanism of action, topical administration, exposed to light corresponding to the absorption spectrum, prevents cell proliferation. Consequently, it results in the destruction of cancer cells. The objective of this work is to investigate the effectiveness of the new natural detector Turmeric Longa L. (CLL), as a response to incident radiation in the treatment of cancer. It has an irradiation system composed of a blue LED lamp. Samples at five-minute doses from 1.54 kJ/cm2 to 30.85 kJ/cm2 and ten-minute doses from 3.08 kJ/cm2 to 61.71 kJ/cm2, produced solutions (CLL-C1/CLL-C2) and irradiated in triplicates. Subsequently, UV-Vis spectrophotometry as an evaluation method, absorbance readings, as well as Design of Experiment (DOE), Linear Regression Multiple (RLM), Mean Squared Percentage Error (RMSPE) and Percentage Error Medium (MSPE). In conclusion, the methods are suitable to be validated in determination of interactions in temperature, light and Concentrations. Keywords Photodynamic Therapy; Design of Experiment; New Natural Detector.