Apostila de técnicas básicas em Microbiologia: cultivo, semeadura, identificação e crescimento

Abstract

This material presents a structured technical-didactic guide for teaching fundamental techniques in Microbiology, ranging from biosafety regulations to quantitative methods of cellular analysis. The content is organized into practical lesson plans and techniques for guidance on culture media preparation, sterilization, isolation of environmental microorganisms, and inoculation methods. Furthermore, it addresses essential antimicrobial susceptibility testing procedures and microscopy protocols focused on the morphological identification of bacteria, fungi, and protozoa. In the final stage, the material delves into the study of metabolism and microbial growth kinetics, detailing quantification methods such as spectrophotometry, colony-forming unit (CFU) counting, and direct microscopy. This content constitutes an essential resource for the standardization of laboratory practices, aiming to develop technical and scientific skills in the microbiological field.