Manual prático para análise de produção enzimática por microrganismos: teste de triagem em placas

Abstract

This manual presents technical guidelines for prospecting and analyzing enzyme production by microorganisms, highlighting the use of qualitative solid media methods as a screening strategy. The theoretical framework addresses the importance of microorganisms in the synthesis of bioproducts and enzymes of industrial interest, detailing how cultivation in Petri dishes allows the detection of specific catalytic activities. The methodology focuses on qualitative assays where enzyme production is evidenced by the formation of degradation halos around microbial colonies following the addition of specific indicators or revealing reagents. The guide meticulously describes the necessary materials and operational procedures for conducting the tests, providing a standardized basis for selecting isolates with biotechnological potential for substrate conversion and bioprocess development.