Manual prático para obtenção de metabólitos secundários produzidos por microrganismos: cultivo submerso e cultivo em estado sólido

Abstract

This manual integrates the fundamentals of microbial biotechnology with the practical procedures required for the utilization of biomolecules of industrial interest. The material explores how microorganisms can be manipulated in a laboratory environment for the synthesis of secondary metabolites, highlighting the importance of optimizing physicochemical variables to enhance the yield of these metabolic pathways. On the experimental side, the focus lies on submerged cultivation, continuously covering the cycle that begins with fermentation and ends in downstream processing. Through recovery and concentration protocols, the guide demonstrates how to transform the cultivated medium into purified crude extracts, serving as a guiding tool for the standardization of experiments aimed at extracting bioactive compounds and developing new bioproducts.