Extração e quantificação de flavonoides por análise espectrofotométrica UV/VIS no mastruz (chenopodium ambrosioides L.)

Abstract

The demand for medicinal plants for the prevention and treatment of diseases of various types has increased considerably in recent years.The use of plants for therapeutic purposes has wide popular acceptance and support from the World Health Organization (WHO), motivating scientific research.This type of alternative therapy has been sought to combat diseases with low cost and easy access. Among the plants with medicinal potential is mastruz (Chenopodium ambrosioides L.). According to the literature, in Brazilian popular medicine, mastruz is used in the treatment of wounds, inflammatory processes, as an anthelmintic and in the treatment of problems of the respiratory system through juices and infusions. The present work was characterized as an experimental analytical study, where methanol and ethanol were evaluated as solvents in the extraction and quantification of flavonoids in mastruz through spectrophotometry in the visible ultraviolet region (UV/VIS), using rutin, as a comparative standard. The results obtained from the analyzed solutions, expressed in mg rutin equivalent per milliliter (mg ER/mL), showed that the flavonoid content obtained was 5.13 mg ER/mL, in the solution of the mastruz sprayed with metanol (0.102%); 4.18 mg ER/mL, in the solution of the mastruz sprayed with etanol (0.084%); 4.88 mg ER/mL, in the solution of the mastruz macerated with metanol (0.098%); and 3.95 mg ER/mL, in the solution of the matruz macerated with etanol (0.079%). With the analysis, it was concluded that methanol was the solvent with the best performance in the extraction of flavonoids in the mastruz, both in the solution where the pulverized mastruz was used, and in the solution in which the macerated mastruz was used.