Imobilização de α-amilase em magnetita

Abstract

The immobilization of biocatalysts improves their stability in the face of degrading conditions and generates a great optimization in terms of cost, by enabling their reuse. The α–amylases have wide application from food production to applicability in the textile area and molecular biology. In this context, magnetite is a good support for enzymatic immobilization because it performs a good enzyme-support interaction through molecular interactions. The present work had as its main purpose the immobilization of α-amylase, using magnetite, where its synthesis, by coprecipitation, demonstrated magnetic activity when exposed to a magnet and a yield of 6.14%. The immobilization of α-amylase presents an activity of 0.66 EU/mL for 30 min of reaction, and 0.70 EU/mL for 60 min and immobilization efficiency was obtained, respectively, of 47.1% and 49.9 %, using the physical adsorption methodology.