Otimização da técnica extração sólido-líquido com partição em baixa temperatura para determinação de carbofurano em Cucurbita Pepo L (“abobrinha”) por cromatografia líquida de alta eficiência

Abstract

The consumption of pesticides in the world is growing every year, and Brazil has a prominent position in this ranking. The indiscriminate use of these compounds promote environmental contamination, bringing severe impacts on fauna, flora and water resources. Another aspect should be monitored refers to the residues of these pesticides in food, and that should be a point of constant vigilance to maintain food quality and safety of the individual. The aim of the study was to optimize the analytical process of the art solid-liquid extraction with partition at low temperature for carbofuran extraction in arrays of Cucurbita pepo L ("zucchini"). The plant in question was chosen due to the results obtained in the last survey of the Pesticide Residue Analysis Program on Food of the National Health Surveillance Agency (FOR) for the year 2012 and published in 2014. For optimization was employed a factorial design complete 2³ to assess the simultaneous performance of three factors on two levels, in triplicate. For separation, identification and quantification of carbofuran was used high-performance liquid chromatography with ultraviolet detector (HPLC-UV). The solid-liquid partition extraction technique with Low Temperature (NGE / PBT) optimized for the zucchini proved simple and efficient, consuming a small quantity of sample and solvent extractor. The percentage of average recovery was 75.88%. Samples of courgettes acquired in trade Itumbiara-GO and submitted to the optimized technique showed no carbofuran residues.