https://repositorio.ifg.edu.br/handle/prefix/2584 Tue, 06 Oct 2026 22:22:46 GMT 2026-10-06T22:22:46Z https://repositorio.ifg.edu.br/handle/prefix/2588 Título: Manual prático para análise do potencial antagonista microbiano: teste de confronto ou antagonismo Autor(es): Chagas, Ronney; Saiki, Patricia Abstract: This practical manual provides guidelines for analyzing the antagonistic potential of microorganisms, aiming to identify biological control agents as a sustainable alternative to the indiscriminate use of pesticides in agriculture. The theoretical framework addresses the environmental and public health impacts caused by synthetic chemicals, highlighting biological control as an essential tool in Integrated Pest Management (IPM). The methodology details the confrontation test, or dual culture method, using Potato Dextrose Agar (PDA) medium to observe pathogen growth inhibition through mechanisms such as antibiosis, mycoparasitism, and competition. The material describes inoculation procedures, incubation, and the quantification of biocontrol efficacy through the calculation of the Percentage of Radial Growth Inhibition (PIR). This guide seeks to standardize the laboratory screening of microbial isolates with potential for developing robust agricultural bioproducts. Editor: Instituto Federal de Educação, Ciência e Tecnologia de Goiás Tipo: Relatórios Técnicos Ciêntíficos Thu, 01 Jan 2026 00:00:00 GMT https://repositorio.ifg.edu.br/handle/prefix/2588 2026-01-01T00:00:00Z https://repositorio.ifg.edu.br/handle/prefix/2587 Título: Apostila de técnicas básicas em Microbiologia: cultivo, semeadura, identificação e crescimento Autor(es): Chagas, Ronney; Saiki, Patricia Abstract: This material presents a structured technical-didactic guide for teaching fundamental techniques in Microbiology, ranging from biosafety regulations to quantitative methods of cellular analysis. The content is organized into practical lesson plans and techniques for guidance on culture media preparation, sterilization, isolation of environmental microorganisms, and inoculation methods. Furthermore, it addresses essential antimicrobial susceptibility testing procedures and microscopy protocols focused on the morphological identification of bacteria, fungi, and protozoa. In the final stage, the material delves into the study of metabolism and microbial growth kinetics, detailing quantification methods such as spectrophotometry, colony-forming unit (CFU) counting, and direct microscopy. This content constitutes an essential resource for the standardization of laboratory practices, aiming to develop technical and scientific skills in the microbiological field. Editor: Instituto Federal de Educação, Ciência e Tecnologia de Goiás Tipo: Relatórios Técnicos Ciêntíficos Thu, 01 Jan 2026 00:00:00 GMT https://repositorio.ifg.edu.br/handle/prefix/2587 2026-01-01T00:00:00Z https://repositorio.ifg.edu.br/handle/prefix/2586 Título: Manual prático para análise de produção enzimática por microrganismos: teste de triagem em placas Autor(es): Chagas, Ronney; Saiki, Patricia Abstract: This manual presents technical guidelines for prospecting and analyzing enzyme production by microorganisms, highlighting the use of qualitative solid media methods as a screening strategy. The theoretical framework addresses the importance of microorganisms in the synthesis of bioproducts and enzymes of industrial interest, detailing how cultivation in Petri dishes allows the detection of specific catalytic activities. The methodology focuses on qualitative assays where enzyme production is evidenced by the formation of degradation halos around microbial colonies following the addition of specific indicators or revealing reagents. The guide meticulously describes the necessary materials and operational procedures for conducting the tests, providing a standardized basis for selecting isolates with biotechnological potential for substrate conversion and bioprocess development. Editor: Instituto Federal de Educação, Ciência e Tecnologia de Goiás Tipo: Relatórios Técnicos Ciêntíficos Thu, 01 Jan 2026 00:00:00 GMT https://repositorio.ifg.edu.br/handle/prefix/2586 2026-01-01T00:00:00Z https://repositorio.ifg.edu.br/handle/prefix/2585 Título: Manual prático para obtenção de metabólitos secundários produzidos por microrganismos: cultivo submerso e cultivo em estado sólido Autor(es): Chagas, Ronney; Saiki, Patricia Abstract: This manual integrates the fundamentals of microbial biotechnology with the practical procedures required for the utilization of biomolecules of industrial interest. The material explores how microorganisms can be manipulated in a laboratory environment for the synthesis of secondary metabolites, highlighting the importance of optimizing physicochemical variables to enhance the yield of these metabolic pathways. On the experimental side, the focus lies on submerged cultivation, continuously covering the cycle that begins with fermentation and ends in downstream processing. Through recovery and concentration protocols, the guide demonstrates how to transform the cultivated medium into purified crude extracts, serving as a guiding tool for the standardization of experiments aimed at extracting bioactive compounds and developing new bioproducts. Editor: Instituto Federal de Educação, Ciência e Tecnologia de Goiás Tipo: Relatórios Técnicos Ciêntíficos Thu, 01 Jan 2026 00:00:00 GMT https://repositorio.ifg.edu.br/handle/prefix/2585 2026-01-01T00:00:00Z